Facilities

WATERS Acquity UPLC H-Class, Coupled with Acquity-

TQD MS/MS

Charges per sample/type of analysis:

For Industries/commercial companies: Rs. 5000+ GST per sample per profile.

For Govt. R & D labs: Rs 3000 + GST per sample per profile

For students: Rs 1500 + GST per sample per profile. Only top 10 compounds of the chromatograph will be identified or the mass will be given.

Mr. Tapas Kumar Roy, Division of Plant Physiology and Biochemistry,Chief Technical Officer. Email:tkr@iihr.res.in, Ph: +91-80-23086100 – Ext 247

Specifications:

Pressure can be hold up-to 15000 psi, with flow from 0.05 ml to 0.5 ml/min PDA Detector 190nm -900nm

MS/MS Mass range scan 20amu to 2000 amu, with electro spray ionization of ESI+ and ESI-, APCI+ and APCI-

Highlights of importance and applications of instruments:

To measure the molecular wt. and their fragmentation of unknown compounds. Plants Metabolomics and phytochemicals identifications and quantification by injecting through UPLC or by direct insertion to MS/MS.

Parameters/analysis that can be analysed in the instrument::

Profiling and estimation of Phenolic acids, Flavonoids, Amino acids, Polyamines, Water and Fat soluble vitamins, Plant Hormones, Organic acids, Anthocyanins, Alkaloids, Saponins, Carotenoids, Sugars etc.

User instructions for sample preparation:

Extraction should be done as per the standard published methodology for individual groups of compounds. A minimum of 2 ml of the extract can be sent for the LC-MS analysis. If solid samples (Plant samples/mushrooms only) are to be sent then a minimum of 100 g is needed.